Postdoctoral Researcher

Shozo Honda

Postdoctoral Researcher

Short Bio

Shozo joined Yohei Kirino's Lab in 2011 as a postdoctoral researcher. He specializes in biochemistry and molecular biology. His research is focused on the biogenesis and function of PIWI interacting RNA (piRNA) and tRNA functions in cancer. He received his PhD from Tokyo Medical and Dental University (Johji Inazawa Lab), Japan. During his PhD studies, he developed an original BAC-based X-tiling array and discovered novel pathogenic copy number variations (CNVs) related to X-linked mental retardation (XLMR).

Publications

2016

  • Izumi, N, Shoji, K, Sakaguchi, Y, Honda, S, Kirino, Y, Suzuki, T, Katsuma, S, Tomari, Y. Identification and Functional Analysis of the Pre-piRNA 3" Trimmer in Silkworms. Cell. 2016;164 (5):962-73. doi: 10.1016/j.cell.2016.01.008. PubMed PMID:26919431 PubMed Central PMC4856147.
  • Honda, S, Morichika, K, Kirino, Y. Selective amplification and sequencing of cyclic phosphate-containing RNAs by the cP-RNA-seq method. Nat Protoc. 2016;11 (3):476-89. doi: 10.1038/nprot.2016.025. PubMed PMID:26866791 PubMed Central PMC4852555.

2015

  • Telonis, AG, Loher, P, Honda, S, Jing, Y, Palazzo, J, Kirino, Y, Rigoutsos, I. Dissecting tRNA-derived fragment complexities using personalized transcriptomes reveals novel fragment classes and unexpected dependencies. Oncotarget. 2015;6 (28):24797-822. doi: 10.18632/oncotarget.4695. PubMed PMID:26325506 PubMed Central PMC4694795.
  • Honda, S, Loher, P, Shigematsu, M, Palazzo, JP, Suzuki, R, Imoto, I, Rigoutsos, I, Kirino, Y. Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers. Proc. Natl. Acad. Sci. U.S.A. 2015;112 (29):E3816-25. doi: 10.1073/pnas.1510077112. PubMed PMID:26124144 PubMed Central PMC4517238.
  • Honda, S, Shigematsu, M, Morichika, K, Telonis, AG, Kirino, Y. Four-leaf clover qRT-PCR: A convenient method for selective quantification of mature tRNA. RNA Biol. 2015;12 (5):501-8. doi: 10.1080/15476286.2015.1031951. PubMed PMID:25833336 PubMed Central PMC4615770.
  • Honda, S, Kirino, Y. Dumbbell-PCR: a method to quantify specific small RNA variants with a single nucleotide resolution at terminal sequences. Nucleic Acids Res. 2015;43 (12):e77. doi: 10.1093/nar/gkv218. PubMed PMID:25779041 PubMed Central PMC4499115.
  • Londin, E, Loher, P, Telonis, AG, Quann, K, Clark, P, Jing, Y, Hatzimichael, E, Kirino, Y, Honda, S, Lally, M, Ramratnam, B, Comstock, CE, Knudsen, KE, Gomella, L, Spaeth, GL, Hark, L, Katz, LJ, Witkiewicz, A, Rostami, A, Jimenez, SA, Hollingsworth, MA, Yeh, JJ, Shaw, CA, McKenzie, SE, Bray, P, Nelson, PT, Zupo, S, Van Roosbroeck, K, Keating, MJ, Calin, GA, Yeo, C, Jimbo, M, Cozzitorto, J, Brody, JR, Delgrosso, K, Mattick, JS, Fortina, P, Rigoutsos, I. Analysis of 13 cell types reveals evidence for the expression of numerous novel primate- and tissue-specific microRNAs. Proc. Natl. Acad. Sci. U.S.A. 2015;112 (10):E1106-15. doi: 10.1073/pnas.1420955112. PubMed PMID:25713380 PubMed Central PMC4364231.

2014

2013

  • Honda, S, Kirino, Y, Maragkakis, M, Alexiou, P, Ohtaki, A, Murali, R, Mourelatos, Z, Kirino, Y. Mitochondrial protein BmPAPI modulates the length of mature piRNAs. RNA. 2013;19 (10):1405-18. doi: 10.1261/rna.040428.113. PubMed PMID:23970546 PubMed Central PMC3854531.

2012

  • Suzuki, R, Honda, S, Kirino, Y. PIWI Expression and Function in Cancer. Front Genet. 2012;3 :204. doi: 10.3389/fgene.2012.00204. PubMed PMID:23087701 PubMed Central PMC3472457.
  • Honda, S, Hayashi, S, Nakane, T, Imoto, I, Kurosawa, K, Mizuno, S, Okamoto, N, Kato, M, Yoshihashi, H, Kubota, T, Nakagawa, E, Goto, Y, Inazawa, J. The incidence of hypoplasia of the corpus callosum in patients with dup (X)(q28) involving MECP2 is associated with the location of distal breakpoints. Am. J. Med. Genet. A. 2012;158A (6):1292-303. doi: 10.1002/ajmg.a.35321. PubMed PMID:22528406.
  • Honda, S, Satomura, S, Hayashi, S, Imoto, I, Nakagawa, E, Goto, Y, Inazawa, J, Japanese Mental Retardation Consortium. Concomitant microduplications of MECP2 and ATRX in male patients with severe mental retardation. J. Hum. Genet. 2012;57 (1):73-7. doi: 10.1038/jhg.2011.131. PubMed PMID:22129561.

2011

  • Hayashi, S, Imoto, I, Aizu, Y, Okamoto, N, Mizuno, S, Kurosawa, K, Okamoto, N, Honda, S, Araki, S, Mizutani, S, Numabe, H, Saitoh, S, Kosho, T, Fukushima, Y, Mitsubuchi, H, Endo, F, Chinen, Y, Kosaki, R, Okuyama, T, Ohki, H, Yoshihashi, H, Ono, M, Takada, F, Ono, H, Yagi, M, Matsumoto, H, Makita, Y, Hata, A, Inazawa, J. Clinical application of array-based comparative genomic hybridization by two-stage screening for 536 patients with mental retardation and multiple congenital anomalies. J. Hum. Genet. 2011;56 (2):110-24. doi: 10.1038/jhg.2010.129. PubMed PMID:20981036.

2010

  • Honda, S, Hayashi, S, Imoto, I, Toyama, J, Okazawa, H, Nakagawa, E, Goto, Y, Inazawa, J. Copy-number variations on the X chromosome in Japanese patients with mental retardation detected by array-based comparative genomic hybridization analysis. J. Hum. Genet. 2010;55 (9):590-9. doi: 10.1038/jhg.2010.74. PubMed PMID:20613765.
  • Honda, S, Orii, KO, Kobayashi, J, Hayashi, S, Imamura, A, Imoto, I, Nakagawa, E, Goto, Y, Inazawa, J. Novel deletion at Xq24 including the UBE2A gene in a patient with X-linked mental retardation. J. Hum. Genet. 2010;55 (4):244-7. doi: 10.1038/jhg.2010.14. PubMed PMID:20339384.

2007

  • Hayashi, S, Honda, S, Minaguchi, M, Makita, Y, Okamoto, N, Kosaki, R, Okuyama, T, Imoto, I, Mizutani, S, Inazawa, J. Construction of a high-density and high-resolution human chromosome X array for comparative genomic hybridization analysis. J. Hum. Genet. 2007;52 (5):397-405. doi: 10.1007/s10038-007-0127-4. PubMed PMID:17406783.
  • Honda, S, Hayashi, S, Kato, M, Niida, Y, Hayasaka, K, Okuyama, T, Imoto, I, Mizutani, S, Inazawa, J. Clinical and molecular cytogenetic characterization of two patients with non-mutational aberrations of the FMR2 gene. Am. J. Med. Genet. A. 2007;143A (7):687-93. doi: 10.1002/ajmg.a.31638. PubMed PMID:17343270.

Contact Info

Email: Shozo.Honda@jefferson.edu